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pparg flox flox  (Jackson Laboratory)
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Jackson Laboratory pparg flox flox
A. Schematic overview of the strategy we used to study how BAT affects systemic metabolite and lipid profiles in vivo. Step 1, generating the BAT ablation mouse model ( Ucp1 -Cre; <t>Pparg</t> <t>flox/flox</t> , herein BAT-mice) and littermate control ( Pparg flox/flox , herein BAT+ mice); Step 2, collecting the serum samples from these mice under acute cold (6 °C for 1 and 3 h), chronic cold (6 °C for 3 days), and thermoneutrality (28 °C for 3 days), with ad libitum feeding; Step 3, quantifying the dynamic profiles of metabolites and lipids across different temperature conditions by LC-MS; Step 4, selecting the metabolites and lipids altered by BAT ablation and clustering them based on their profile similarity. The altered molecules included those accumulated in BAT-ablated mice, which might be cleared by BAT, and those elevated in mice with intact BAT, which might be derived from BAT. N = 8 for BAT-ablated mice and N = 10 for littermate controls. B-C. Correlation clustering of circulating metabolites ( B ) and lipids ( C ) altered by BAT ablation. An unpaired t-test was used to determine the significance of each circulating metabolite or lipid between BAT+ and BAT- mice under each temperature condition. A total of 206 metabolites and 249 lipids with p < 0.05 in at least one condition were included for correlation clustering. Clustering analysis was performed using Mfuzz package in R software, and pairwise Pearson correlation coefficients between molecules were used to generate heatmaps. Table S1 lists the p values and fold changes between the BAT+ and BAT- groups for these metabolites and lipids. N = 8 for BAT-ablated mice and N = 10 for littermate controls. BAT- > BAT+, molecules were accumulated in BAT-ablated mice. BAT+ > BAT-, molecules were elevated in littermate controls with intact BAT. BAT-, BAT-ablated mice; BAT+, littermate controls. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. Mice were provided with ad libitum access to food and water throughout the experiment. AMP, adenosine monophosphate; γ-Glu-Glu, glutamyl-glutamic acid; Pyro-Glu, pyroglutamic acid, SAM, S-adenosylmethionine; GSSG, oxidized glutathione; GSH, reduced glutathione; GABA, γ-aminobutyric acid; TG, triglyceride; DG, diglyceride; PE, phosphatidylethanolamine; PC, phosphatidylcholine; SM, sphingomyelin; Cer, ceramide; EtherPE, ether-linked phosphatidylethanolamine; EtherPC, ether-linked phosphatidylcholine; CE, cholesterol ester; LPE, lysophosphatidylethanolamine; LPC, lysophosphatidylcholine; LNAPE, N-acyl-lysophosphatidylethanolamine; HexCer, hexosylceramide; CAR, acylcarnitine; OHFA, hydroxy fatty acid; DiOHFA, dihydroxy fatty acid; DHA, docosahexaenoic acid. D-E. Representative quantitative profiles of known BAT-regulated metabolites ( D ) and lipids ( E ). Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. F. Representative quantitative profiles of metabolites and lipids newly identified as BAT-regulated in this study. Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. G. Relative quantitative profiles of indicated fatty acids and 12, 13-diHOME in serum from BAT-ablated mice and littermate controls. Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days.
Pparg Flox Flox, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. Schematic overview of the strategy we used to study how BAT affects systemic metabolite and lipid profiles in vivo. Step 1, generating the BAT ablation mouse model ( Ucp1 -Cre; Pparg flox/flox , herein BAT-mice) and littermate control ( Pparg flox/flox , herein BAT+ mice); Step 2, collecting the serum samples from these mice under acute cold (6 °C for 1 and 3 h), chronic cold (6 °C for 3 days), and thermoneutrality (28 °C for 3 days), with ad libitum feeding; Step 3, quantifying the dynamic profiles of metabolites and lipids across different temperature conditions by LC-MS; Step 4, selecting the metabolites and lipids altered by BAT ablation and clustering them based on their profile similarity. The altered molecules included those accumulated in BAT-ablated mice, which might be cleared by BAT, and those elevated in mice with intact BAT, which might be derived from BAT. N = 8 for BAT-ablated mice and N = 10 for littermate controls. B-C. Correlation clustering of circulating metabolites ( B ) and lipids ( C ) altered by BAT ablation. An unpaired t-test was used to determine the significance of each circulating metabolite or lipid between BAT+ and BAT- mice under each temperature condition. A total of 206 metabolites and 249 lipids with p < 0.05 in at least one condition were included for correlation clustering. Clustering analysis was performed using Mfuzz package in R software, and pairwise Pearson correlation coefficients between molecules were used to generate heatmaps. Table S1 lists the p values and fold changes between the BAT+ and BAT- groups for these metabolites and lipids. N = 8 for BAT-ablated mice and N = 10 for littermate controls. BAT- > BAT+, molecules were accumulated in BAT-ablated mice. BAT+ > BAT-, molecules were elevated in littermate controls with intact BAT. BAT-, BAT-ablated mice; BAT+, littermate controls. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. Mice were provided with ad libitum access to food and water throughout the experiment. AMP, adenosine monophosphate; γ-Glu-Glu, glutamyl-glutamic acid; Pyro-Glu, pyroglutamic acid, SAM, S-adenosylmethionine; GSSG, oxidized glutathione; GSH, reduced glutathione; GABA, γ-aminobutyric acid; TG, triglyceride; DG, diglyceride; PE, phosphatidylethanolamine; PC, phosphatidylcholine; SM, sphingomyelin; Cer, ceramide; EtherPE, ether-linked phosphatidylethanolamine; EtherPC, ether-linked phosphatidylcholine; CE, cholesterol ester; LPE, lysophosphatidylethanolamine; LPC, lysophosphatidylcholine; LNAPE, N-acyl-lysophosphatidylethanolamine; HexCer, hexosylceramide; CAR, acylcarnitine; OHFA, hydroxy fatty acid; DiOHFA, dihydroxy fatty acid; DHA, docosahexaenoic acid. D-E. Representative quantitative profiles of known BAT-regulated metabolites ( D ) and lipids ( E ). Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. F. Representative quantitative profiles of metabolites and lipids newly identified as BAT-regulated in this study. Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. G. Relative quantitative profiles of indicated fatty acids and 12, 13-diHOME in serum from BAT-ablated mice and littermate controls. Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days.

Journal: bioRxiv

Article Title: BAT protects against hepatic oxidative stress by remodeling the circulating metabolome

doi: 10.64898/2026.05.12.722834

Figure Lengend Snippet: A. Schematic overview of the strategy we used to study how BAT affects systemic metabolite and lipid profiles in vivo. Step 1, generating the BAT ablation mouse model ( Ucp1 -Cre; Pparg flox/flox , herein BAT-mice) and littermate control ( Pparg flox/flox , herein BAT+ mice); Step 2, collecting the serum samples from these mice under acute cold (6 °C for 1 and 3 h), chronic cold (6 °C for 3 days), and thermoneutrality (28 °C for 3 days), with ad libitum feeding; Step 3, quantifying the dynamic profiles of metabolites and lipids across different temperature conditions by LC-MS; Step 4, selecting the metabolites and lipids altered by BAT ablation and clustering them based on their profile similarity. The altered molecules included those accumulated in BAT-ablated mice, which might be cleared by BAT, and those elevated in mice with intact BAT, which might be derived from BAT. N = 8 for BAT-ablated mice and N = 10 for littermate controls. B-C. Correlation clustering of circulating metabolites ( B ) and lipids ( C ) altered by BAT ablation. An unpaired t-test was used to determine the significance of each circulating metabolite or lipid between BAT+ and BAT- mice under each temperature condition. A total of 206 metabolites and 249 lipids with p < 0.05 in at least one condition were included for correlation clustering. Clustering analysis was performed using Mfuzz package in R software, and pairwise Pearson correlation coefficients between molecules were used to generate heatmaps. Table S1 lists the p values and fold changes between the BAT+ and BAT- groups for these metabolites and lipids. N = 8 for BAT-ablated mice and N = 10 for littermate controls. BAT- > BAT+, molecules were accumulated in BAT-ablated mice. BAT+ > BAT-, molecules were elevated in littermate controls with intact BAT. BAT-, BAT-ablated mice; BAT+, littermate controls. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. Mice were provided with ad libitum access to food and water throughout the experiment. AMP, adenosine monophosphate; γ-Glu-Glu, glutamyl-glutamic acid; Pyro-Glu, pyroglutamic acid, SAM, S-adenosylmethionine; GSSG, oxidized glutathione; GSH, reduced glutathione; GABA, γ-aminobutyric acid; TG, triglyceride; DG, diglyceride; PE, phosphatidylethanolamine; PC, phosphatidylcholine; SM, sphingomyelin; Cer, ceramide; EtherPE, ether-linked phosphatidylethanolamine; EtherPC, ether-linked phosphatidylcholine; CE, cholesterol ester; LPE, lysophosphatidylethanolamine; LPC, lysophosphatidylcholine; LNAPE, N-acyl-lysophosphatidylethanolamine; HexCer, hexosylceramide; CAR, acylcarnitine; OHFA, hydroxy fatty acid; DiOHFA, dihydroxy fatty acid; DHA, docosahexaenoic acid. D-E. Representative quantitative profiles of known BAT-regulated metabolites ( D ) and lipids ( E ). Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. F. Representative quantitative profiles of metabolites and lipids newly identified as BAT-regulated in this study. Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days. G. Relative quantitative profiles of indicated fatty acids and 12, 13-diHOME in serum from BAT-ablated mice and littermate controls. Relative units: quantitative peak areas from LC-MS normalized to the average. N = 8 for BAT-ablated mice and N = 10 for littermate controls. Statistic: unpaired t-test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Ad libitum conditions. C-1h, 6 °C for 1 hour; C-3h, 6 °C for 3 hours; C-3d, 6 °C for 3 days; TN-3d, 28 °C for 3 days.

Article Snippet: PPARg flox/flox (Stock No. 004584), and Ucp1 -Cre (Stock No. 024670) mice were obtained from the Jackson Laboratory.

Techniques: In Vivo, Control, Liquid Chromatography with Mass Spectroscopy, Derivative Assay, Software